Ohashi Lab.
東北大学大学院 生命科学研究科
分子化学生物学専攻 ケミカルバイオロジー講座 分子細胞生物分野
東北大学理学部化学科 生物化学教室
Laboratory of Molecular and Cellular Biology
Department of Biomolecular Sciences
Graduate School of Life Sciences
Tohoku University
原著論文・総説
2021年
1. Ninomiya K., Ohta K., Yamashita K., Mizuno K.*, Ohashi K.*
PLEKHG4B enables actin cytoskeletal remodeling during epithelial cell-cell junction formation.
J. Cell Sci., 134, (2021) jcs249078 *) Co-corresponding authors
2020年
1. Khan L., Sato K., Okuyama S., Kobayashi T., Ohashi K., Hirasaka K., Nikawa T, Takada K., Higashitani A., Abiko K.
Ultra-high-purity iron is a novel and very compatible biomaterial.
J. Mech. Behav. Biomed. Mater., 106, 103744 (2020)
2. Isozaki Y., Sakai K., Kohiro K., Kagoshima K., Iwamura Y., Sato H., Rindner D., Fujiwara S., Yamashita K., Mizuno K., Ohashi K.*
The Rho-guanine nucleotide exchange factor Solo decelerates collective cell migration by modulating the Rho-ROCK pathway and keratin networks.
Mol. Cell Biol., 31: 741-752 (2020) DOI: 10.1091/mbc.E19-07-0357 *) Corresponding author
3. 大橋一正
細胞競合と力覚応答
医学のあゆみ, 274: 525-530 (2020) DOI:10.1111/gtc.12682
2019年
1. Sato D.X., Ishii Y., Nagai T., Ohashi K., Kawata M.
Human-specific mutations in VMAT1 confer functional changes and multi-directional evolution in the regulation of monoamine circuits.
BMC Evol. Biol., 19: 220 (2019) DOI:10.1186/s12862-019-1543-8.
2. Fujiwara S., Matsui T.S., Ohashi K., Mizuno K., Deguchi S.
Keratin-binding ability of the N-terminal Solo domain of Solo is critical for its function in cellular mechanotransduction.
Genes Cells, 24: 390-402 (2019) DOI:10.1111/gtc.12682
3. 山下和成,磯崎友亮,鹿子嶋克彦,國富 葵,大橋一正(共著、1項目)
細胞の力覚応答におけるRho-GEFの機能
細胞, 51: 687-690 (2019) review
2018年
1. Nishimura R., Kato K., Fujiwara S., Ohashi K.*, Mizuno K.*
Solo and keratin filaments regulate epithelial tubule morphology.
Cell Struc. Funct., 43: 95-105 (2018) DOI:10.1247/csf.18010 *) Co-corresponding authors
2. Fujiwara S., Matsui T.S., Ohashi K., Deguchi S., Mizuno K.
Solo, a RhoA-targeting guanine nucleotide exchange factor, is critical for hemidesmosome formation and acinar development in epithelial cells.
PLoS One, 13: e0195124 (2018) DOI:10.1371/journal.pone.0195124
2017年
1. Ohashi K.*, Fujiwara S., Mizuno K.
Roles of the cytoskeleton, cell adhesion and rho signalling in mechanosensing and mechanotransduction.
J. Biochem. 161: 245-254 (2017) review *) Corresponding author
2016年
1. Fujiwara S., Ohashi K.*, Mashiko T., Kondo H., Mizuno K.*
Interplay between Solo and keratin filaments is crucial for mechanical force-induced stress fiber reinforcement.
Mol. Biol. Cell, 27: 954-966 (2016) *) Co-corresponding authors
2015年
1. Abiko H., Fujiwara S., Ohashi K.*, Hiatari R., Mashiko T., Sakamoto N., Sato M., Mizuno K.*
Rho-guanine nucleotide exchange factors involved in cyclic stretch-induced reorientation of vascular endothelial cells.
J. Cell Sci. 128: 1683-1695 (2015) *) Co-corresponding authors
2. Ohashi K.
Roles of cofilin in development and its mechanisms of regulation.
Develop. Growth Differ. 57: 275-290 (2015) review
2014年
1. Homma Y., Kanno S., Sasaki K., Nishita M., Yasui A., Asano T., Ohashi K., Mizuno K.
Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation.
J. Biol. Chem. 289: 26302-26313 (2014)
2. Ohashi K.*, Sampei K., Nakagawa M., Uchiumi N., Amanuma T., Aiba S., Oikawa M., Mizuno K*.
Damnacanthal, an effective inhibitor of LIM-kinase, inhibits cell migration and invasion.
Mol. Biol. Cell, 25: 828-840 (2014) *) Co-corresponding authors
3. Ohashi K.* and Mizuno K. (共著、1項目)
A novel pair of split Venus fragments to detect protein–protein interactions by in vitro and in vivo bimolecular fluorescence complementation assays.
Methods in Molecular Biology, 1174: 247-262, Editor: Ivanov AI, Springer, (2014) Book
*) as corresponding author
2013年
1. Hou X., Katahira T., Ohashi K., Mizuno K., Sugiyama S., Nakamura H.
Coactosin accelerates cell dynamism by promoting actin polymerization.
Dev. Biol., 379: 53-63 (2013)
2. Saito A., Miyajima K., Akatsuka J., Kondo H., Mashiko T., Kiuchi T., Ohashi K.*, Mizuno K.*
CaMKIIβ-mediated LIM-kinase activation plays a crucial role in BDNF-induced neuritogenesis.
Genes Cells, 18: 533-543 (2013) *) Co-corresponding authors
3. Hayashi A., Hiatari R., Tsuji T., Ohashi K., Mizuno K.
p63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration in breast carcinoma cells.
FEBS Lett. 587: 698-705 (2013)
2012年
1. Ikeda M., Chiba S., Ohashi K., Mizuno K.
Furry Protein Promotes Aurora A-mediated Polo-like Kinase 1 Activation.
J. Biol. Chem. 287: 27670-27681 (2012)
2. Shoji K., Ohashi K., Sampei K., Oikawa M., Mizuno K.
Cytochalasin D acts as an inhibitor of the actin-cofilin interaction.
Biochem. Biophys. Res. Commun. 424: 52-57 (2012)
3. Ohashi K., Kiuchi T., Shoji K., Sampei K., Mizuno K.
Visualization of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence complementation assays using a new pair of split Venus fragments.
Biotechniques 52: 45-50 (2012)
4. 大橋一正、辻 拓史、水野健作(共著、1項目)
「Wntの細胞内平面極性シグナルによるRhoファミリーの活性化とアクチン細胞骨格制御機構」
生体の科学, 63: 177-182 医学書院 (2012) review
2011年
1. Ohashi K.*, Fujiwara S., Watanabe T., Kondo H., Kiuchi T., Sato M., Mizuno K.*
LIM-kinase has a dual role in regulating lamellipodium extension by decelerating the rate of actin retrograde flow and the rate of actin polymerization.
J. Biol. Chem. 286: 36340-36351 (2011) *) Co-corresponding authors
2. Kiuchi T., Nagai T., Ohashi K., Mizuno K.
Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension.
J. Cell Biol. 193: 365-380 (2011)
3. Kiuchi T., Nagai T., Ohashi K., Watanabe N., Mizuno K.
Live-cell imaging of G-actin dynamics using sequential FDAP.
BioArchitecture, 1: 240-244 (2011) review
2010年
1. Tsuji T., OhtaY., Kanno Y., Hirose K., Ohashi K., Mizuno K.
Involvement of p114-RhoGEF and Lfc in Wnt-3a- and Dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells.
Mol. Biol. Cell 21: 3590-3600 (2010)
2. Mishima T., Naotsuka M., Horita Y., Sato M., Ohashi K., Mizuno K.
LIM-kinase is critical for the mesenchymal-to-amoeboid cell morphological transition in 3D matrices.
Biochem. Biophys. Res. Commun. 392: 577-581 (2010)